FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a1.351000000a1db2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a1.351000000a1db2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997677
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315890.24303573630888045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146100.11240470124007544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA47400.011.67576534-35
ACCCATA56350.011.5466886
CCCATAA53300.011.405487
CGGGAAT39600.010.7960531
CGGGGAT34900.010.7527461
CGTATGC38450.010.44052644-45
TATGCCG38950.010.18472546-47
CGGCAAT38900.010.1355241
CCATAAA70100.010.0270038
CGCGAAT25150.09.8216181
AAAAAAC200550.09.6639611
CGGAAAT33600.09.6136281
AGAGCAC90200.09.5827988
AAAAACC129250.09.5553992
GAGCACA91300.09.4672349
ACGTCTG42800.09.43182914-15
CTCGTAT43800.09.38144542-43
CACGAAT35500.09.3667161
GTATGCC43000.09.33577244-45
GGCGAAT42550.09.2660841
Produced by FastQC (version 0.11.2)