FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb72_tsu-0_r2o_hn.3510000008977e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb72_tsu-0_r2o_hn.3510000008977e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18104045
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG992340.5481316468225748TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG132900.038.4787615
GCACACG136300.037.91506211
TCGTATG133400.037.4069545
GTCACGT133400.037.2226729
ACGTGAA136950.036.322632
ACACGTC141500.036.3150213
AGTCACG138550.036.1639528
CACACGT143700.035.884312
AGCACAC144900.035.68028310
TCACGTG140200.035.59293430
CTCGTAT141550.035.3008644
CACGTGA142200.034.997431
CACGTCT150100.034.17439314
AACGATC147750.033.53022438
CGTGAAA148150.033.5006233
GAGCACA156250.033.3188449
TCGGAAG155900.033.167793
ACGATCT152500.032.47108539
CGTCTGA160850.031.83448416
TCTCGTA157500.031.8259443
Produced by FastQC (version 0.11.2)