FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb70_stw-0_r2o_hn.351000000896f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb70_stw-0_r2o_hn.351000000896f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21475683
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1172430.5459337428290406TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC128650.041.81372533
ACGTCCG132950.040.5801832
CCGCACA136150.039.642136
GTCCGCA136050.039.53894434
CGCACAT139700.038.68304437
ACGTCTG146200.037.73263515
GCACACG146350.037.72470511
TCCGCAC145000.037.1449835
GTCACGT147000.036.70339229
AGTCACG151400.035.978628
TCGTATG150750.035.86249545
ACACGTC154850.035.6248713
AGCACAC159300.034.81329710
CACACGT160550.034.4441612
TCACGTC159700.034.00834330
CTCGTAT159700.033.97945444
CACGTCC160650.033.8212431
CACGTCT166450.033.19621714
GCACATC168200.032.31580438
TCGGAAG172200.032.1966933
Produced by FastQC (version 0.11.2)