FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb68_st-0_r2o_hn.3510000008967f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb68_st-0_r2o_hn.3510000008967f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17346318
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG863410.49774828294973034TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG112650.037.94635815
GCACACG118450.036.25922811
TCGTATG116600.035.79258345
TATCTCG119650.035.03072441
AGCACAC124950.034.44501510
ACACGTC125650.034.1098813
CTCGTAT124050.033.71555344
CACACGT128600.033.397412
CACGTCT128550.033.37538514
CGTCTGA133900.031.94105316
TCGGAAG135000.031.5517443
GAGCACA141000.030.7794469
GATCGGA139250.030.6466431
CAGTCAC139100.030.44082827
TCTCGTA139000.030.26735943
ATCGGAA143700.029.6730022
AGAGCAC149000.028.8701698
GTCACAT146950.028.67681529
CCTTTAT146550.028.585337
CGGAAGA151050.028.571264
Produced by FastQC (version 0.11.2)