FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb66_shahdara_r2o_hn.351000000895ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb66_shahdara_r2o_hn.351000000895ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24095923
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC1413770.5867258124953337TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC166850.040.72252733
CACCCGT172350.039.4623231
ACGTCTG177450.038.9862415
GTATGCC174700.038.74998545
TCACCCG176250.038.55081630
ACCCGTC177900.038.2439332
GCACACG181900.038.143811
CGTATGC181850.037.17691844
GTCACCC187200.036.44188329
CACACGT191750.036.2195912
ACACGTC192200.036.16990713
AGCACAC192850.036.16465410
TCGTATG188050.035.99905443
CTCGTAT190850.035.44740342
CACGTCT199850.034.7290814
CCGTCCA196950.034.3952834
GAGCACA205100.033.9936839
TCGGAAG205200.033.806463
CGTCTGA208500.033.35302416
CAGTCAC213400.032.25256327
Produced by FastQC (version 0.11.2)