FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb64_sakata_r2o_hn.35100000089563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb64_sakata_r2o_hn.35100000089563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22534295
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1598650.7094297824715616TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG192450.038.94045315
GCACACG194700.038.54822511
TCGTATG193400.037.9232445
ACACGTC199150.037.66427213
CACACGT204200.036.75484512
CTCGTAT202450.036.1946444
AGCACAC209650.035.96034610
CACGTCT214650.034.9445114
GAGCACA216700.034.831969
CGTCTGA218100.034.39174316
ACATGTC215450.034.3768732
TCGGAAG219700.034.1662333
GATCGGA222400.033.724651
ATGTCAG219250.033.6060134
TCTCGTA222650.033.1331743
CAGTCAC225500.032.99596827
GTCAGAA224400.032.94510736
TGTCAGA223650.032.8945635
GTCACAT224650.032.86026829
CACATGT226950.032.6348531
Produced by FastQC (version 0.11.2)