FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb62_oy-0_r2o_hn.351000000894de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb62_oy-0_r2o_hn.351000000894de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814095
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC329020.30425107232736537TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG41050.034.966733
ACGTCTG43350.034.2531115
GTATGCC42600.033.90553745
CGTATGC45200.032.1045444
GCACACG47400.031.37388211
TCGTATG47050.030.6987443
ACTAATC47500.030.64515732
CACACGT49550.030.05795912
ACACGTC50350.029.53569213
CTCGTAT49500.029.3611142
CACTAAT51150.028.76625331
AGTCACT52350.028.06517628
AGCACAC53550.027.89675910
CACGTCT53950.027.56482114
TCGGAAG57700.025.8155613
GTCACTA57150.025.5505229
GAGCACA59800.025.507849
GATCGGA59550.024.9645141
TCTCGTA58950.024.84519241
CGTCTGA60550.024.67170116
Produced by FastQC (version 0.11.2)