FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb60_n13_r2o_hn.35100000089464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb60_n13_r2o_hn.35100000089464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33522351
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1985730.5923600048218575TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG250950.038.56812715
GCACACG254450.038.22329711
TCGTATG255450.036.7085445
CACACGT264750.036.6937412
AGCACAC267450.036.28965810
ACACGTC267350.036.22749313
CACGTCT277600.034.94657514
CTCGTAT268800.034.90214544
GAGCACA285600.034.1094749
ACAGTCA276350.033.96579732
CACAGTC276450.033.9128231
GTCACAG282000.033.3580329
TCGGAAG299100.032.42373
CGTCTGA299650.032.37500416
CAGTCAC293350.032.24387427
ACTCCAG305650.031.53520423
TCTCGTA303000.031.1854543
TCACAGT302500.031.11139530
AGAGCAC312750.031.0764758
TCCAGTC314250.030.71514725
Produced by FastQC (version 0.11.2)