FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb58_mt-0_r2o_hn.351000000893ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb58_mt-0_r2o_hn.351000000893ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28669693
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1644490.5735987476391882TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG202600.038.87743415
GTATGCC199550.038.80646545
CGTATGC209550.036.95456744
TCGTATG210500.036.8732943
GCACACG214900.036.80928811
ACACGTC216450.036.50411613
CTCGTAT222350.034.86780542
CACACGT229450.034.53394312
AGCACAC235500.033.69453410
GTCACCT232100.033.47251529
CACGTCT236500.033.38083314
GAGCACA239950.033.275939
TCGGAAG241850.032.758783
CGTCTGA247700.031.90781616
TCTCGTA245450.031.59546341
GATCGGA251100.031.4961051
ATCGGAA253800.031.1810552
AATCTCG250400.031.01584839
CAGTCAC255150.030.68672827
AGAGCAC259500.030.6649748
Produced by FastQC (version 0.11.2)