FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l08n01_nutrinetat_vegb56_mr-0_r2o_hn.35100000089365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l08n01_nutrinetat_vegb56_mr-0_r2o_hn.35100000089365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20464492
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2080101.016443506147135TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC238900.041.25795733
GTATGCC239450.041.09671845
ACGGCTA241050.040.9929332
ACGTCTG247100.040.6074115
GCACACG249150.040.4177711
CGTATGC243150.040.4158344
AGTCACG247950.040.1350328
GGCTACA245200.040.09638234
TCGTATG248700.039.53243
CACGGCT252050.039.23961631
CACACGT257550.039.0995412
AGCACAC258450.039.05043810
ACACGTC258000.038.96158213
GTCACGG255400.038.73515329
GCTACAT255250.038.4912235
CTCGTAT259250.037.9407242
TCACGGC261200.037.8994830
CTACATC260700.037.8246336
GAGCACA270350.037.46479
CACGTCT268950.037.3920414
Produced by FastQC (version 0.11.2)