FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb54_mh-1_r2o_hn.35100000089761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb54_mh-1_r2o_hn.35100000089761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18948680
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1135070.5990232565012443TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG142500.038.5391315
GCACACG145000.038.200611
AGTCACG147150.036.7128
CACACGT151000.036.637912
TCACGTG147250.036.57811430
GTCACGT146800.036.5369829
TCGTATG147550.036.4882145
ACGTGAA148150.036.41655332
ACACGTC152250.036.26321413
CACGTGA150050.035.9705231
AGCACAC154150.035.94769310
CTCGTAT153300.035.2957144
AACGATC156700.034.18629538
CGTGAAA157750.034.18604333
CACGTCT160950.034.17723514
GAGCACA165650.033.58799
TCGGAAG166750.032.8714563
ACGATCT165850.032.36805739
TCTCGTA168750.032.18418543
CGTCTGA171600.032.1216516
Produced by FastQC (version 0.11.2)