FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb52_kn-0_r2o_hn.351000000896e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb52_kn-0_r2o_hn.351000000896e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13049812
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG827640.6342160331505159TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC90650.042.41557733
ACGTCCG93300.041.3555332
GTCCGCA95650.040.1745234
CCGCACA97750.039.3347536
CGCACAT98000.039.2344137
GCACACG101550.039.0147511
GTCACGT100600.038.3993129
ACGTCTG102900.038.37156715
TCCGCAC100550.038.2840135
AGTCACG105200.036.805828
ACACGTC108400.036.38316313
AGCACAC109450.036.2398210
TCGTATG106650.036.22073445
CACACGT110650.035.8466612
TCACGTC108450.035.64057530
CACGTCC109550.035.4059331
CTCGTAT110400.035.1330644
CACGTCT113000.034.90207714
GAGCACA116300.034.3374569
GCACATC112550.034.30227338
Produced by FastQC (version 0.11.2)