FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb50_ge-0_r2o_hn.35100000089662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb50_ge-0_r2o_hn.35100000089662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19576397
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1758720.8983879924380365TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG210150.040.17897411
ACGTCTG210100.039.95274415
TCGTATG212500.039.06743245
ACACGTC218350.038.6183613
TATCTCG215550.038.55697341
AGCACAC219450.038.54800410
CACACGT221150.038.18027512
CTCGTAT222100.037.3889244
CACGTCT226150.037.22670714
GAGCACA235050.036.2384839
TCGGAAG233850.035.8230863
GATCGGA236900.035.2615781
CAGTCAC239000.034.9347127
TCTCGTA238400.034.91748443
CCTTTAT238800.034.8877937
CGTCTGA241700.034.7851516
ATCGGAA241800.034.6173672
ATCTCGT243750.034.2162242
ACTCCAG247900.033.9889923
AGAGCAC251200.033.8549238
Produced by FastQC (version 0.11.2)