FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb48_edi-0_r2o_hn.351000000895f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb48_edi-0_r2o_hn.351000000895f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19100170
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC1042090.5455920025842702TruSeq Adapter, Index 16 (98% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297840.15593578486474205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC126000.040.10405733
CACCCGT129250.039.20038231
TCACCCG131650.038.43448630
ACGTCTG135800.038.0878115
GTATGCC133100.038.0659945
GCACACG137200.037.89593511
ACCCGTC135100.037.55282232
GTCACCC135950.037.4009729
CGTATGC136800.036.9870944
ACACGTC144450.036.04065313
CACACGT145550.035.81464412
AGCACAC146100.035.74141710
TCGTATG142750.035.5872743
CCGTCCA145550.034.6244334
CTCGTAT149000.034.01946342
CACGTCT154050.033.75087714
GAGCACA164100.032.0677459
TCGGAAG163250.031.7292063
CGTCTGA164100.031.67015616
GATCGGA164100.031.5137251
Produced by FastQC (version 0.11.2)