FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb46_ct-1_r2o_hn.35100000089557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb46_ct-1_r2o_hn.35100000089557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23102762
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1110270.48057890221091315TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG145500.037.4967115
GCACACG146600.037.30743811
TCGTATG146250.036.04299545
ACACGTC156950.034.76120813
CACACGT158300.034.5216112
CTCGTAT159250.033.31262244
AGCACAC165450.033.22011610
CACGTCT169350.032.20266714
GAGCACA171100.032.162589
CGTCTGA171300.031.90175616
ACATGTC171800.030.99732632
ATGTCAG171750.030.94051734
TCGGAAG175200.030.9014133
GATCGGA178450.030.1935391
CAGTCAC180950.029.80361427
AGAGCAC186600.029.4909848
GTCAGAA179300.029.45021636
CACATGT183800.029.24291831
ATCGGAA184650.029.2102662
TGTCAGA181850.029.20995335
Produced by FastQC (version 0.11.2)