FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb44_col-0_r2o_hn.351000000894c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb44_col-0_r2o_hn.351000000894c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15618619
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC820460.5253089277611548TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG98850.039.19305433
GTATGCC100800.038.7473345
ACGTCTG105950.037.4583715
GCACACG107300.037.15482711
ACTAATC105950.036.75785432
CGTATGC106700.036.4992544
CACACGT111600.035.74339712
CTCGTAT110550.035.33067742
ACACGTC115250.034.65043313
CACTAAT113500.034.64970831
TCGTATG112750.034.60061343
AGTCACT114200.034.3783328
AGCACAC116300.034.27956410
CACGTCT116800.034.0942914
TCGGAAG121400.032.4170763
TCTCGTA120950.032.18112641
GAGCACA127850.031.4291029
GATCGGA126350.031.0183141
CGTCTGA128450.030.9319916
CAGTCAC131750.030.03835927
Produced by FastQC (version 0.11.2)