FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb42_bur-0_r2o_hn.3510000008944b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb42_bur-0_r2o_hn.3510000008944b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28007826
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2391930.8540220151324848No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1550160.5534738754803746TruSeq Adapter, Index 13 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420070.5070261433357948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG201400.038.45023315
GCACACG205850.037.65181411
TCGTATG205750.036.3688845
CACACGT215750.035.99710512
AGCACAC216950.035.90169510
ACACGTC216000.035.82003413
CACGTCT222700.034.80299414
CTCGTAT223900.033.5714444
GAGCACA234400.033.353769
ACAGTCA223950.033.27278532
GTCACAG231950.032.154329
CACAGTC232200.032.0809231
CGTCTGA242750.031.95623416
CAGTCAC238000.031.53567327
TCGGAAG244200.031.485833
GATCGGA249050.030.8327391
TCTCGTA245000.030.71691543
ATCGGAA254650.030.1672442
AGAGCAC258950.030.0961068
ACTCCAG256550.030.0454323
Produced by FastQC (version 0.11.2)