FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb40_bi-1_r2o_hn.351000000893c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb40_bi-1_r2o_hn.351000000893c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25619756
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1899770.7415254071896703TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC218650.040.33516345
GCACACG230500.039.13022611
ACGTCTG230700.039.0865515
CGTATGC226800.038.8162944
ACACGTC238500.037.80824713
TCGTATG236950.037.30547343
CACACGT243150.037.1407212
AGCACAC245800.036.7677610
CTCGTAT245350.036.06556742
CACGTCT250050.036.02586714
GTCACCT250250.035.40392329
TCGGAAG254100.035.0944023
GAGCACA262650.034.528899
CGTCTGA266000.033.9840816
CAGTCAC264350.033.65203527
GATCGGA266350.033.5394481
TCTCGTA266100.033.24478541
ATCGGAA269400.033.059532
AATCTCG271250.032.52235439
AGAGCAC282200.032.1847238
Produced by FastQC (version 0.11.2)