FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l07n01_nutrinetat_vegb38_akita_r2o_hn.3510000008934c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l07n01_nutrinetat_vegb38_akita_r2o_hn.3510000008934c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20647656
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1800830.8721716402094262TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC207950.041.09069433
GTATGCC210600.040.39203645
ACGGCTA213000.040.2009832
GCACACG217600.040.15750511
ACGTCTG218900.039.89846415
CGTATGC215650.039.42519444
GGCTACA216850.039.2796534
AGCACAC223850.039.11669510
AGTCACG220300.038.991528
CACACGT226650.038.62352812
ACACGTC227050.038.4861213
CACGGCT224300.038.20578831
GTCACGG224600.038.19482429
TCGTATG223650.038.04512843
TCACGGC225700.037.9189630
CTCGTAT225500.037.68366642
GCTACAT227900.037.45419335
CACGTCT235500.037.07653414
CTACATC234950.036.2638336
TCGGAAG240200.036.10353
Produced by FastQC (version 0.11.2)