FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb71_tsu-0_r2y_hn.3510000008972e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb71_tsu-0_r2y_hn.3510000008972e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22471387
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG978830.43558948986993995TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG131300.037.4730315
GTCACGT133450.036.19779229
GCACACG138850.035.53272211
TCGTATG137800.034.97083345
AGTCACG139800.034.81111528
CTCGTAT139300.034.57803744
CACACGT143350.034.448612
ACGTGAA141150.034.31693632
AGCACAC147350.033.7425510
ACACGTC146150.033.7116613
TCACGTG146250.033.09124830
CACGTGA148050.032.81049731
CACGTCT153450.032.07858714
CGTGAAA153100.031.61032933
AACGATC153500.031.35241538
TCTCGTA156350.031.09590743
GAGCACA160700.030.9954179
TCGGAAG160950.030.7299183
ACGATCT157900.030.46450639
GATCGGA164400.029.9397321
Produced by FastQC (version 0.11.2)