FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb69_stw-0_r2y_hn.351000000896a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb69_stw-0_r2y_hn.351000000896a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17329370
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1022830.5902291889433949TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC115200.042.4383933
ACGTCCG120050.040.76160432
GTCCGCA121450.040.3654834
CCGCACA122250.040.06463636
CGCACAT124050.039.55583237
ACGTCTG127700.039.04062315
GTCACGT128950.038.05471829
GCACACG131100.038.04539511
TCCGCAC131000.037.3713935
CTCGTAT133100.036.91581344
TCGTATG133450.036.8864345
AGTCACG135600.036.43735528
ACACGTC137750.036.3719413
AGCACAC139050.035.9672810
CACACGT140350.035.6500612
TCACGTC139150.035.21671730
CACGTCT143100.034.83918814
GCACATC141400.034.6227238
CACGTCC142300.034.46877331
GAGCACA149000.033.927829
Produced by FastQC (version 0.11.2)