FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb67_st-0_r2y_hn.35100000089612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb67_st-0_r2y_hn.35100000089612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21580858
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG835440.3871208457050225TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG107150.036.4703215
TCGTATG116000.033.222545
GCACACG117850.033.17822611
ACACGTC119950.032.6910713
CTCGTAT118100.032.5364344
TATCTCG119000.032.36830541
AGCACAC123550.031.9388910
CACACGT123750.031.7599412
CACGTCT126700.030.96719414
TCGGAAG137350.028.8337063
TCTCGTA135150.028.73212443
GAGCACA139000.028.5992579
CGTCTGA140500.027.94158716
GATCGGA142100.027.6548271
ATCGGAA145750.027.0638662
CAGTCAC146350.026.7502427
CCTTTAT149000.026.06326137
CTGAACT151750.025.89984519
GTCACAT150150.025.8933729
CGGAAGA155150.025.5391834
Produced by FastQC (version 0.11.2)