FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb65_shahdara_r2y_hn.3510000008959a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb65_shahdara_r2y_hn.3510000008959a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22330486
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC1301770.5829564121443662TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC155000.040.65815433
GTATGCC160550.039.12390545
ACGTCTG166550.038.7140515
CACCCGT163450.038.6521431
ACCCGTC163550.038.61328532
TCACCCG168250.037.4960330
GCACACG173900.037.32357811
CACACGT176250.036.78763612
CGTATGC170600.036.7268144
CTCGTAT173750.036.19126542
ACACGTC179600.036.0889313
AGCACAC181750.035.76104410
TCGTATG176400.035.6219943
GTCACCC178500.035.46894529
CACGTCT186600.034.57837714
TCTCGTA188900.033.4687641
TCGGAAG195550.033.3128243
GAGCACA196200.033.2763379
GATCGGA198800.032.7022251
CCGTCCA195050.032.33280634
Produced by FastQC (version 0.11.2)