FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb63_sakata_r2y_hn.35100000089513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb63_sakata_r2y_hn.35100000089513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18570459
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG981360.5284522046547153TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG125200.037.82534415
GCACACG125550.037.77365511
CACACGT133100.035.61406712
ACACGTC134000.035.40844713
CTCGTAT132200.035.10764344
TCGTATG133000.034.86273245
AGCACAC137150.034.66081610
CACGTCT139050.034.18720214
ATGTCAG141800.033.0028534
ACATGTC142200.032.98743432
TGTCAGA141600.032.95448335
GAGCACA146800.032.520299
CACATGT145250.032.3588931
TCTCGTA144400.032.2200843
TCGGAAG147850.032.1732373
GTCAGAA145900.032.01408436
CGTCTGA150150.031.80971116
GATCGGA152350.031.1697441
ATCGGAA154900.030.6362972
CATGTCA153750.030.554933
Produced by FastQC (version 0.11.2)