FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb61_oy-0_r2y_hn.351000000894b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb61_oy-0_r2y_hn.351000000894b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226914
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC126230.5668382344356361TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG14000.039.69573633
ACGTCTG14450.039.07885415
GTATGCC14750.037.521445
ACTAATC15050.037.07243332
AGCACAC15150.036.9762310
GCACACG15500.036.86701211
CTCGTAT15150.036.82938842
CACTAAT15350.036.3487131
TCGTATG15250.036.29281643
ACACGTC15700.035.96747613
CGTATGC15500.035.560744
AGTCACT16150.034.54815328
CACACGT16600.034.2884912
CACGTCT16850.033.37920814
TCGGAAG17450.032.3676953
TCTCGTA17250.032.3479941
ATCGGAA17850.031.6423662
GAGCACA18100.031.57129
GATCGGA18100.031.5254731
CAGTCAC18300.030.735127
Produced by FastQC (version 0.11.2)