FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb59_n13_r2y_hn.3510000008943e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb59_n13_r2y_hn.3510000008943e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29488028
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1762320.5976391503697704TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG225350.038.1764511
ACGTCTG226050.037.8790215
CACACGT230400.037.261512
AGCACAC232500.036.95403710
CTCGTAT233350.036.26982544
TCGTATG233700.036.2059445
ACACGTC241450.035.46304713
CACAGTC245400.034.52815231
ACAGTCA245800.034.44298632
CACGTCT252150.034.02062614
TCGGAAG255950.033.6639333
GAGCACA256100.033.6540839
GTCACAG255900.033.14663329
TCTCGTA260850.032.5587143
GATCGGA264950.032.551051
CAGTCAC263500.032.25042327
ATCGGAA267100.032.2333682
CGTCTGA269800.031.82005316
TCACAGT273850.031.05613530
ACTCCAG278250.030.87955723
Produced by FastQC (version 0.11.2)