FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb57_mt-0_r2y_hn.351000000893b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb57_mt-0_r2y_hn.351000000893b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34638459
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2145960.619531024749109TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC252300.039.3955345
ACGTCTG259900.038.840715
GCACACG271150.037.33738711
CGTATGC269100.036.9443144
TCGTATG270500.036.76168443
AGCACAC278300.036.56405610
CTCGTAT273700.036.3483242
ACACGTC279900.036.20201513
CACACGT282850.035.82444412
GTCACCT285550.034.96838829
CACGTCT296700.034.07632414
GAGCACA301450.033.7859579
TCGGAAG300550.033.6915863
TCTCGTA301500.033.0574541
GATCGGA317250.031.9978921
AATCTCG316050.031.67106639
ATCGGAA320800.031.6630572
CAGTCAC317900.031.57985527
CGTCTGA321950.031.48064416
CTGAACT332750.030.31690219
Produced by FastQC (version 0.11.2)