FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l06n01_nutrinetat_vegb55_mr-0_r2y_hn.3510000008933f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l06n01_nutrinetat_vegb55_mr-0_r2y_hn.3510000008933f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20577348
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1529530.7433076410040789TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC180300.040.6047533
GTATGCC182050.040.31163445
ACGTCTG186200.040.07761415
ACGGCTA188050.039.1937832
GCACACG193450.038.6687411
CGTATGC189000.038.6387244
GGCTACA189450.038.5961334
GTCACGG194350.038.1336929
TCGTATG192550.038.09038543
AGCACAC198150.037.81966410
CACACGT198950.037.61095412
AGTCACG196900.037.60564428
ACACGTC198700.037.5903413
CTCGTAT197650.037.14177342
GCTACAT197650.037.1205435
CACGGCT199350.037.0304331
TCACGGC200350.036.9130730
CTACATC201350.036.2372836
CACGTCT207600.035.9246214
TCGGAAG211400.035.403243