FastQCFastQC Report
Mon 17 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb53_mh-1_r2y_hn.35100000089711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb53_mh-1_r2y_hn.35100000089711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24321533
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG2744011.1282224685425872TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG322550.041.27021415
GTCACGT323350.040.30611829
GCACACG331150.040.26097511
ACGTGAA326550.040.04615832
AGTCACG327750.039.88859628
ACACGTC335700.039.7876213
TCACGTG327900.039.77427330
CACACGT336850.039.61171312
TCGTATG329100.039.50545545
CTCGTAT330000.039.43861844
AGCACAC339250.039.3593810
CACGTGA333600.039.1807531
CACGTCT345950.038.47870614
CGTGAAA340700.038.37224633
AACGATC338550.038.3319238
GAGCACA352250.037.9834449
TCGGAAG354050.037.8558273
CGTCTGA357600.037.23771716
ACGATCT349050.037.23039639
GATCGGA360200.037.1705551
Produced by FastQC (version 0.11.2)