FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb51_kn-0_r2y_hn.35100000089699.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb51_kn-0_r2y_hn.35100000089699.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13207228
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1785661.3520323871140862TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC191100.043.4766733
ACGTCCG194550.042.7924732
CCGCACA196900.042.25312436
GTCCGCA197050.042.16307434
GCACACG202600.041.8426311
CGCACAT199450.041.72419437
ACGTCTG204350.041.43853415
TCCGCAC202200.041.0558135
GTCACGT203650.040.96152529
ACACGTC208950.040.55857513
CACACGT208950.040.55857512
AGCACAC209800.040.41753810
TCGTATG206550.040.40945
AGTCACG208400.040.22226728
CTCGTAT208700.040.02505544
TCACGTC209300.039.81277530
CACGTCC210150.039.660531
CACGTCT214850.039.42385514
GAGCACA217150.039.1325349
GCACATC214300.038.88538738
Produced by FastQC (version 0.11.2)