FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb49_ge-0_r2y_hn.3510000008962f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb49_ge-0_r2y_hn.3510000008962f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18009512
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1697830.9427407028019416TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG202050.040.84166715
GCACACG207400.039.9847211
TCGTATG205100.039.6529645
ACACGTC209500.039.4752113
TATCTCG206100.039.33219541
AGCACAC212600.039.09138510
CACACGT213050.038.933612
CTCGTAT211100.038.48329544
CACGTCT217250.038.0462914
GAGCACA224950.037.0453459
TCGGAAG231700.036.0257843
CAGTCAC228300.035.7974827
TCTCGTA228750.035.64183443
GATCGGA234400.035.564711
CCTTTAT230050.035.50146537
CGTCTGA234150.035.3579116
ATCGGAA236500.035.246942
AGAGCAC241200.034.661578
ATCTCGT234600.034.64029742
GTCACAT236000.034.5436129
Produced by FastQC (version 0.11.2)