FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb47_edi-0_r2y_hn.351000000895a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb47_edi-0_r2y_hn.351000000895a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17829662
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC2087701.1709139522667338TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC229300.042.2774133
CACCCGT235300.041.2527231
GTATGCC237150.041.0575245
GCACACG250750.041.03927211
ACGTCTG250400.041.00529515
TCACCCG236950.040.95837430
ACCCGTC240950.040.312532
CACACGT255750.040.25306312
GTCACCC242900.040.2237229
CGTATGC243550.039.950944
ACACGTC259200.039.69993213
AGCACAC259750.039.64329510
CACGTCT262250.039.1610114
CTCGTAT250050.038.9333342
TCGTATG250750.038.7678743
CCGTCCA253850.038.06401434
TCGGAAG272900.037.908723
GAGCACA273500.037.8065579
GATCGGA274900.037.7271921
CGTCTGA275450.037.28435516
Produced by FastQC (version 0.11.2)