FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb45_ct-1_r2y_hn.35100000089520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb45_ct-1_r2y_hn.35100000089520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22819915
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1560250.6837229674168375TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG190150.039.32731615
GCACACG194900.038.46195211
CACACGT199850.037.5423412
CTCGTAT194550.037.46652644
TCGTATG195650.037.2328845
ACACGTC203200.036.76840613
AGCACAC206550.036.2708910
ACATGTC205250.035.71603432
CACGTCT210500.035.47192814
ATGTCAG207300.035.2272634
TGTCAGA207500.035.19330635
GTCAGAA210600.034.6026136
CACATGT212450.034.58057831
GAGCACA218450.034.418789
TCGGAAG218800.034.2943
TCTCGTA218000.033.61173643
GATCGGA225750.033.2425461
CATGTCA222550.033.07690433
CAGTCAC223700.032.98391327
CGTCTGA227000.032.98276516
Produced by FastQC (version 0.11.2)