FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb43_col-0_r2y_hn.3510000008949b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb43_col-0_r2y_hn.3510000008949b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22824869
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC1209040.5297029306060858TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG137600.039.32205233
GTATGCC140000.038.6472845
ACTAATC145600.037.28846432
ACGTCTG150100.037.17088315
GCACACG153600.036.3987211
CGTATGC151700.035.68140844
CTCGTAT153450.035.42334442
AGCACAC157800.035.38716510
ACACGTC158500.035.2151413
CACACGT158650.035.19602612
TCGTATG155450.034.8930143
CACTAAT157350.034.8056331
AGTCACT160300.034.11067628
CACGTCT165800.033.6103814
GAGCACA175800.031.827899
TCTCGTA172450.031.62488641
TCGGAAG179100.031.2801633
CAGTCAC181700.030.26661527
GATCGGA188400.029.66681
CTGAACT187450.029.57244119
Produced by FastQC (version 0.11.2)