FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb41_bur-0_r2y_hn.35100000089414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb41_bur-0_r2y_hn.35100000089414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18051310
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1216000.6736353206498586TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG148450.039.08390415
GCACACG152750.038.19113511
AGCACAC156300.037.32370410
CACACGT158300.036.73723612
ACACGTC159200.036.5860713
CTCGTAT156450.036.58207744
CACGTCT159500.036.53136414
TCGTATG157850.036.28612545
GAGCACA168150.034.8004389
CACAGTC164700.034.68816431
ACAGTCA166100.034.32710332
TCTCGTA168800.034.07886543
TCGGAAG173200.033.7621233
GTCACAG169850.033.74405729
CAGTCAC176000.032.87187627
AATCTCG174700.032.82683641
CGTCTGA178400.032.62333716
GATCGGA179600.032.564681
ATCGGAA182200.032.180842
ACTCCAG182150.031.9403323
Produced by FastQC (version 0.11.2)