FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb39_bi-1_r2y_hn.3510000008939c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb39_bi-1_r2y_hn.3510000008939c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20753624
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2318531.1171687412280382TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC259700.041.41687845
ACGTCTG265750.040.86340315
GCACACG269850.040.44399311
CGTATGC270200.039.73247544
AGCACAC274750.039.68994510
CACACGT274900.039.6833212
ACACGTC274450.039.6418213
CTCGTAT272300.039.4108642
TCGTATG276250.038.9356143
GTCACCT279000.038.6252829
CACGTCT284650.038.2529314
TCGGAAG290500.037.57973
GAGCACA291950.037.3825579
TCTCGTA288350.037.31862341
GATCGGA293650.037.1508031
CAGTCAC294300.036.6248827
ATCGGAA298550.036.6040082
CGTCTGA300900.036.1870916
TCCAGTC305100.035.51667825
CTGAACT307550.035.42658219
Produced by FastQC (version 0.11.2)