FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l05n01_nutrinetat_vegb37_akita_r2y_hn.35100000089315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l05n01_nutrinetat_vegb37_akita_r2y_hn.35100000089315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18390646
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1875451.0197847318685815TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC214200.041.36096233
GTATGCC215300.041.15931345
ACGGCTA218800.040.6205332
ACGTCTG224850.040.35166515
GGCTACA219400.040.3286334
CGTATGC220050.040.21972344
GCACACG226900.040.0878811
AGTCACG226200.039.58367528
AGCACAC231600.039.26474810
GTCACGG228550.039.24558629
CTCGTAT225800.039.207242
CACACGT232500.039.14007612
ACACGTC234050.038.80397813
TCACGGC230800.038.66789230
CACGGCT230750.038.65466331
TCGTATG229650.038.5580243
GCTACAT230100.038.3946235
CTACATC233700.037.75668736
CACGTCT241150.037.6801614
GAGCACA243700.037.4999439
Produced by FastQC (version 0.11.2)