FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb36_tsu-0_r2o_ln.35100000089605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb36_tsu-0_r2o_ln.35100000089605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23256063
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG3498741.5044420889296697TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG411500.042.09844615
GCACACG412300.042.0385811
GTCACGT405700.041.53144529
TCGTATG410250.041.2616245
ACGTGAA410150.041.22788632
AGTCACG415650.041.0258228
AGCACAC423900.040.93092310
TCACGTG412850.040.89919330
CACACGT424350.040.88195412
ACACGTC424050.040.8631313
CACGTGA414850.040.72834831
CTCGTAT416150.040.7035744
CACGTCT434050.039.88540314
AACGATC423450.039.7947538
CGTGAAA425600.039.68358233
TCGGAAG441450.039.505863
GAGCACA440450.039.4801989
CGTCTGA444250.039.16207516
TCTCGTA434200.039.12549243
GATCGGA447250.039.013971
Produced by FastQC (version 0.11.2)