FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb34_stw-0_r2o_ln.3510000008958d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb34_stw-0_r2o_ln.3510000008958d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19037041
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1565790.8224965213869109TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC168900.043.2510433
ACGTCCG174600.041.91649232
GTCCGCA175650.041.58885634
CCGCACA178600.041.10368336
CGCACAT180200.040.70126737
TCCGCAC182000.040.11309435
ACGTCTG185800.040.10397715
GCACACG186000.040.09714511
GTCACGT184050.039.7533329
AGTCACG190150.038.7639528
ACACGTC192650.038.66633213
TCGTATG191700.038.34156845
AGCACAC198050.037.73711810
CACACGT198850.037.57388312
TCACGTC196350.037.2630530
CTCGTAT198950.037.03481744
CACGTCT201350.036.97327814
CACGTCC199650.036.73622531
GCACATC201450.036.4748938
TCGGAAG208550.036.0890583
Produced by FastQC (version 0.11.2)