FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb32_st-0_r2o_ln.35100000089506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb32_st-0_r2o_ln.35100000089506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14584405
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1643381.1268063386884826TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG189750.041.4034415
GCACACG192500.040.7885911
TCGTATG190350.040.51677745
AGCACAC198000.039.7011610
ACACGTC198150.039.6823213
TATCTCG194650.039.61016541
CACACGT199350.039.4208812
CACGTCT201000.039.09727514
CTCGTAT198600.038.8900544
TCGGAAG210100.037.8030553
CGTCTGA211100.037.45049316
GATCGGA212400.037.4418951
GAGCACA210750.037.4167379
ATCGGAA215400.036.8415572
CAGTCAC212550.036.66928527
TCTCGTA212300.036.36982743
AGAGCAC219450.036.148668
CGGAAGA222250.035.9390264
GTCACAT215100.035.79364429
ACTCCAG220050.035.76627323
Produced by FastQC (version 0.11.2)