FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb30_shahdara_r2o_ln.3510000008948e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb30_shahdara_r2o_ln.3510000008948e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22551925
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC2196510.9739789397135721TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC247950.041.51191733
CACCCGT251550.041.1149631
ACGTCTG260900.040.8050215
TCACCCG255500.040.3838130
ACCCGTC255700.040.3331932
CGTATGC258900.040.06876844
GTATGCC258350.040.05827745
GCACACG267650.039.8263711
GTCACCC262600.039.4375929
ACACGTC271400.039.2429213
CACACGT272350.039.155612
TCGTATG266350.038.9902543
CCGTCCA267650.038.46463834
AGCACAC278500.038.33958410
CGTCTGA280850.038.21086516
CTCGTAT272550.038.14465342
CACGTCT281100.037.8567414
GAGCACA287200.037.2017639
TCGGAAG293100.036.8246963
GATCGGA299700.036.0882841
Produced by FastQC (version 0.11.2)