FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb28_sakata_r2o_ln.35100000089407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb28_sakata_r2o_ln.35100000089407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20139236
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1946150.9663474820991222TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG232050.040.788215
GCACACG237750.039.8954811
TCGTATG235300.039.68948745
ACACGTC239300.039.62766613
AGCACAC246350.038.5119710
CACACGT247250.038.37168512
CTCGTAT246350.037.90912644
CACGTCT252150.037.5992514
GAGCACA254250.037.403919
TCGGAAG257450.037.0641023
CGTCTGA258650.036.8109316
ACATGTC254950.036.6923232
ATGTCAG255200.036.68264434
GATCGGA261250.036.633561
CAGTCAC261350.036.08154727
ATCGGAA266250.035.838982
TCTCGTA263000.035.7914743
GTCAGAA262850.035.6578336
AGAGCAC270500.035.431388
CACATGT265100.035.33847831
Produced by FastQC (version 0.11.2)