FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb26_oy-0_r2o_ln.3510000008938f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb26_oy-0_r2o_ln.3510000008938f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4776362
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC236020.4941417756861812TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG28600.038.86027533
GTATGCC30000.037.8717245
ACGTCTG30650.037.2887815
GCACACG30750.037.2406811
TCGTATG31150.036.32911743
CGTATGC31600.035.95416344
ACTAATC31800.035.3035532
ACACGTC32500.035.23541313
CTCGTAT32550.034.76657542
AGCACAC33350.034.13533410
CACACGT33600.034.0818712
AGTCACT33300.033.58098628
CACTAAT33900.033.2496831
CACGTCT35250.032.55037714
TCTCGTA36800.030.8125541
GAGCACA38150.030.4891649
GTCACTA36800.030.44796829
TCGGAAG37750.030.3972663
AGAGCAC38150.030.0173788
CGTCTGA38600.030.01683416
Produced by FastQC (version 0.11.2)