FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb24_n13_r2o_ln.35100000089308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb24_n13_r2o_ln.35100000089308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20413450
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1479690.7248603249328261TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG182550.039.01868411
ACGTCTG182450.038.90442715
TCGTATG185200.037.95015745
CACACGT192350.036.97224412
AGCACAC193550.036.78951610
ACACGTC196750.036.1454213
CTCGTAT196250.035.77895444
CACGTCT199550.035.7058914
GAGCACA205850.034.7224129
TCGGAAG207200.034.652013
CACAGTC204550.034.4923931
ACAGTCA205150.034.4353832
GTCACAG205700.034.22416729
CGTCTGA209500.034.0745116
GATCGGA211300.034.02841
ATCGGAA216400.033.230812
CAGTCAC213600.033.15981727
AGAGCAC217100.033.1407368
AATCTCG216250.032.5637941
TCTCGTA216850.032.44232643
Produced by FastQC (version 0.11.2)