FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb22_mt-0_r2o_ln.351000000892d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb22_mt-0_r2o_ln.351000000892d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21248141
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1168350.5498598677409002TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC143900.039.49255445
ACGTCTG149100.038.44717415
GCACACG155400.037.0911911
CGTATGC156400.036.3361844
TCGTATG159100.035.776143
CACACGT161850.035.6130512
AGCACAC161900.035.58823410
ACACGTC162400.035.40931713
CTCGTAT161550.035.19175742
GTCACCT169950.033.30846829
TCGGAAG174750.033.179793
GAGCACA175150.033.1785939
CACGTCT174150.032.9685514
GATCGGA180950.032.07081
CGTCTGA181300.032.00340716
ATCGGAA183100.031.7895582
TCTCGTA180000.031.78458441
CAGTCAC184100.031.11660827
CGGAAGA190300.030.681464
AATCTCG187450.030.32930839
Produced by FastQC (version 0.11.2)