FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l04n01_nutrinetat_vegb20_mr-0_r2o_ln.351000000892b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l04n01_nutrinetat_vegb20_mr-0_r2o_ln.351000000892b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22363273
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1854700.8293508736400079TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC217400.040.3286345
CGGCTAC216950.040.25691233
GCACACG222600.039.9424211
ACGTCTG222200.039.89282615
ACGGCTA221700.039.59770232
CGTATGC221300.039.57724844
GGCTACA224250.038.90612834
TCGTATG226900.038.62029643
ACACGTC229950.038.5776713
AGTCACG228200.038.50240328
CACACGT233750.037.9601512
CACGGCT231150.037.8914231
AGCACAC236000.037.75076710
TCACGGC232650.037.6384630
GTCACGG233100.037.62379529
GCTACAT235500.037.03835
CTCGTAT239150.036.65161542
TCGGAAG248400.035.9695973
GAGCACA249250.035.906439
CACGTCT247700.035.8404714
Produced by FastQC (version 0.11.2)