FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb8_col-0_r2o_ln.35100000089471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb8_col-0_r2o_ln.35100000089471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17350834
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC941470.5426079230543039TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG119250.040.0597615
GCACACG120500.039.76296211
CTAATCG124450.039.07513833
GTATGCC126450.038.66718345
CACACGT126050.038.12571712
ACACGTC126750.037.61409813
CGTATGC131500.037.11451744
CACGTCT130300.036.6442214
ACTAATC135100.036.2593632
TCGTATG136750.035.77105743
CACTAAT141150.034.91028231
AGCACAC145800.034.68076310
CTCGTAT141100.034.60514542
AGTCACT143450.034.12056728
CGTCTGA142850.033.3747816
TCGGAAG150100.033.305123
GAGCACA153400.032.919019
GTCACTA151000.032.33959229
GATCGGA156050.032.099321
CAGTCAC153600.032.03982527
Produced by FastQC (version 0.11.2)