FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb6_bur-0_r2o_ln.351000000893f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb6_bur-0_r2o_ln.351000000893f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24131287
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1088380.4510244314776912TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG145050.039.09544411
ACGTCTG144650.038.90680315
ACACGTC153150.036.77857213
CACACGT156550.036.33016212
TCGTATG166200.035.20675745
CACGTCT161850.034.81633414
AGCACAC174700.034.51337410
CTCGTAT178800.032.7506544
CGTCTGA174150.032.27513516
GAGCACA187150.032.1936769
ACAGTCA183200.032.1734132
GTCACAG186450.031.84036629
CACAGTC190150.031.05601731
TCGGAAG195250.030.6344833
CAGTCAC195850.030.35895727
GATCGGA197500.030.3174061
TCTCGTA195500.030.19219443
AGAGCAC203350.029.5085668
ATCGGAA204650.029.183792
TCACAGT206150.028.77604730
Produced by FastQC (version 0.11.2)