FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb4_bi-1_r2o_ln.35100000089372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb4_bi-1_r2o_ln.35100000089372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27410798
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2449430.893600397916179TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG295600.042.80996315
GCACACG298650.042.53250511
ACACGTC304650.041.5382413
CACACGT307150.041.394312
GTATGCC321550.040.7347545
CGTATGC326750.040.0659744
CACGTCT317950.039.7931714
TCGTATG337450.038.8285343
AGCACAC345450.038.657710
CTCGTAT341150.038.3747842
GTCACCT346900.037.86403329
CGTCTGA336050.037.60727716
GATCGGA361750.036.8599971
GAGCACA364350.036.8357249
TCGGAAG361700.036.7592963
TCTCGTA363550.036.0960741
CAGTCAC367750.035.76130327
ATCGGAA374150.035.553972
AATCTCG376950.034.7302239
CTGAACT382350.034.70556319
Produced by FastQC (version 0.11.2)