FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb2_akita_r2o_ln.351000000892fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb2_akita_r2o_ln.351000000892fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21218049
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1412930.6659094811214735TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG175400.041.63141315
GCACACG181300.040.5914811
CGGCTAC189200.040.17313433
ACACGTC182300.040.12091413
GTATGCC193300.039.30729345
CACACGT187450.039.2977912
ACGGCTA195800.038.85439732
CGTATGC197250.038.49765444
AGTCACG201800.037.92316428
GGCTACA200200.037.81920634
CACGTCT194100.037.73084314
TCACGGC206200.037.00441430
AGCACAC213200.036.99661610
CACGGCT206550.036.99398431
TCGTATG205450.036.97196643
GTCACGG208500.036.6710129
GCTACAT210000.036.0648335
CTCGTAT211350.035.83446542
CGTCTGA205250.035.60003316
TCGGAAG222900.035.041563
Produced by FastQC (version 0.11.2)